BIO 3170 Lecture 13: TOPIC 13 RNA SPLICING
6 Dec 2016
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Document Summary
Assist and binding of eukaryotic initiation factor e at the cap. 5" capping is coupled with transcription rnmt = transferase. 5" maturation enzymes bind to ctd of rna pol ii so they can efficiently act on third substrate (which is the end of nascent rna) Ce and rnmp bind to ctd to carry out maturation steps. Substrate is 5" end of newly synthesized rna. Rna triphosphates & guanyltransferase activities are in one protein (ce) in some eukaryotes (human) but separate proteins in others (yeast). Post-transcriptional but coupled with ctd of rna pol ii. Enzymatic event = addition of poly a tail at 3" end (approx. Protects rna from exonuclease activity (like 5"cap) of 3" Black box = stop codon, then 3" utr polya machinery needs to know when to add a"s (same for splicing) Protein-rna interaction of cpsf = cleavage poly specification factor.
