BIO 3170 Lecture 4: BIO3170 midterm 1 review 4
20 Dec 2018
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Document Summary
Reversible nucleases capable to interact w dna break rejoin as needed energetically unfavourable. Type 2 top isomerase producesingle strand break energy stored by the enzyme. Dna will swivel around the phosphodiesterase bond opp the break direction dictated by the tension on dna harvest energy from phospho bond then reuse it to reseal. For every 10 bp helicase progress a complete turn of parental dna is needed to prevent torsion. Problem hairpins ssdna tend to pairtogether which creates hairpins. Single stranded dnabinding proteins ssb stabilize 9 preventssdna from pairing up bydisrupting hbonds. Objective: describe asymmetrical nature of replication fork & okazaki fragments. Bacterial chromosome has 1 replicationbubble but 2 replication fork. Dna primase associated wi both dna strands double stranded break allow 2 helices to pass thru before resealed allows. Atp req"d an rna pot synthesize rna primers 40 ntp. 0 need free 3 ott to add ntp help mark repair. 1 for leading us for each okazaki fragment on lagging.
