BPS 3101 Lecture Notes - Lecture 9: 5.8S Ribosomal Rna, Point Mutation, Transposase

• School: University of Ottawa
• Department: Biopharmaceutical sciences
• Course: BPS 3101
• Professor: Linda Bonen

Document Summary

But low-to-mid repetitive dna fraction includes multi-gene families: dispersed i. e. actin genes on different chromosomes. In tandem arrays i. e. rrna genes, globin genes. Variation in rdna (28s+5. 8s+18s) copy number among eukaryotes. Coming from: nonsense mutations or frameshift mutations or point mutation within initiation codon, truncation: part of gene missing because of deletion or rearrangement. Insertion of transposon (or retrotransposon) into coding or regulatory sequences (of duplicated gene) (cid:862)pro(cid:272)essed pseudoge(cid:374)es(cid:863): cdna copy (lacking introns & promoter) integrated back into genome (transcription reverse transcription re-integration) Centromere: in humans, 1500-30,000 copies of 171 bp tandem repeat (alphoid dna) Telo(cid:373)ere: (cid:1005)(cid:1004)(cid:1004)"s (cid:272)opies of ta(cid:374)de(cid:373)l(cid:455) repeated (cid:373)otif ttagg, short 3" o(cid:448)erha(cid:374)g. A portion of dna in animal cells whose density differs from that of the other dna, consisting of short, repeating sequences of nucleotide pairs near the region of the centromere. Microsatellite dna: tandem repeats of short (~2-6) nt stretches.