BPS 4101 Lecture Notes - Lecture 6: Kir6.2, Fasttrack, Impaired Glucose Tolerance
23 Jan 2018
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Snp genotyping: genotyping tech, whole genome vs targeted, microarray based methods are most common. Samples are applied to a bead chip and separated by an intellihyb seal (or gasket). The loaded bead chip is incubated overnight in the illumina hybridization oven. Unhybridized and non-specifically hybridized dna is washed away and the bead chip is prepared for staining and extension. Dna polymerase; a/t same color; c/g same color. If seq has a, then t will be added. The illumina hiscan or iscan system scans the beadchip, using a laser to excite the fluorophore of the single-base extension product on the beads. The scanner records high-resolution images of the light emitted from the fluorophores. Because the micro wells outnumber the distinct bead types, multiple copies of each bead type are present in the array. This built-in redundancy improves robustness and measurement precision.
