CHM 2354 Lecture Notes - Lecture 16: Molar Attenuation Coefficient, Spectrophotometry, Calorimetry
25 May 2015
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Document Summary
Changes the solution"s ability to absorb light. An analytical technique in which the concentration of an analyte is measured by its ability to produce or. Any technieuq that uses light to measure chemical concentrations. A colorimetric method where an instrument is used to determine the amount of analyte in a sample by the sample"s ability or inability to absorb light of a certain wavelength. Light is made up of photons and electromagnetic waves. Light waves consist of perpendicular oscillating electric and magnetic fields: parametes used to describe light are: Wavelength ( ): distance (usually nm) from peak to peak. Frequency (v) in hz: number of complete oscillations (from max to max) that the waves make each second. Energy (e): the energy of one particle of light (proton) is proportional to its frequency. Energy of light (e) wavelength ( ) increases as frequency (v) increases or decreases. Depending on their level of excitation (increases when molecules absorb light energy)
