HSS 2305 Lecture Notes - Lecture 11: Cystic Fibrosis, Muscular Dystrophy, Hemoglobin

• School: University of Ottawa
• Department: Health Sciences
• Course: HSS 2305
• Professor: Francois Cossette

Document Summary

The red blood cell: an example of the plasma membrane structure. Defects on ion channels and transporters as a cause of inherited disease. 4. 8 the red blood cell: an example of the plasma membrane structure. Plasma membrane of the human red blood cell is the most studied and well understood because of these features: Inexpensive to obtain: obtained in large amount from whole blood, easily obtained since they are present as single cells (no need to digest tissues, very simple: no nuclear nor cytoplasmic membrane no contamination of the erythrocyte plasma membrane. The plasma membrane of a red blood cell is obtained after hemolysis (taking up water and swelling) in a hypotonic salt solution. In presence of salt, the cell becomes leaky and hemoglobin flows out of the cell leaving a (cid:858)ghost(cid:859) plasma membrane. Proteins of the erythrocyte plasma membrane can be separated (fractionated) using a polyacrylamide gel electrophoresis (page) in presence of a detergent (sds: sodium dodecyl sulfate).