An electrophysiologist is studying the electrical properties of an isolated squid neuron placed in normal extracellular fluid. She places a recording electrode inside the cell to record the potential difference across the membrane. She records a resting membrane potential of -70 mV.
A. Name three factors that contribute to this resting membrane potential and explain รขยยhowรขยย they contribute to this negative value
She then applies an electric current across the membrane and records a slight change in the membrane potential. When she increases the current and stimulates the cell again, she notices a rapid reverse in the membrane potential to +30 mV followed by a drop in membrane potential to -75 mV before it returns to -70 mV. When she increases the current some more and stimulates the cell again, she records the same electrical pattern that is the same as what was obtained with the previous current that was applied.
B. What name can be given to the small change in membrane potential seen with the first electrical current applied?
C. Given the type of stimulus, what triggered this change in membrane potential?
D. What is the name given to the electrical pattern obtained with the next current used?
E. What allowed this stimulus to work but not the previous one?
F. Explain the type of feedback mechanism that occurs when this pattern was initiated.
G. Describe the cellular mechanisms that account for the rise and then fall in this electrical pattern
H. Why did the pattern remain the same when the highest stimulus was applied?
The research then changes the extracellular solution (making sure to keep the osmolarity the same) so the it contains double the concentration of Ca2+.
I. Would resting membrane potential change? Why /why not?
J. When she stimulated the cell with the highest current, would the shape of the electrical pattern change? Why / why not?
K. Would synaptic transmission change? Explain
