🏷️ LIMITED TIME OFFER: GET 20% OFF GRADE+ YEARLY SUBSCRIPTION →
Log in
HomeClass Notes1,200,000CA670,000

BIO372H5 Lecture Notes - Lecture 2: Okazaki Fragments, Dna Replication, Ribonuclease H

29 views3 pages
hassan_rizvi96
15 Jan 2020
School
UTM
Department
Biology
Course
BIO372H5
Professor
Ho Sung Rhee

For unlimited access to Class Notes, a Class+ subscription is required.

Document Summary

Both strands of dna are synthesized together at the replication fork. Junction between the newly synthesized template strands and the unreplicated duplex dna. Can be replicated continuously as the dna polymerase moves in the same direction of the replication fork. Synthesized in the 5" 3" direction. Directs the dna polymerase to move in the opposite direction of the replication fork. Synthesized in the 5" 3" direction but away from replication fork. Short fragments of new dna formed on the lagging strand. Okazaki fragments are transient intermediates in dna replication. Initiation of a new strand of dna requires an rna primer. These primers are subsequently extended by dna polymerase. Both leading and lagging strands require primase. Primase does not require specific dna sequences to initiate dna synthesis. Primase is activated when it is associated with dna helicase. A protein that unwinds the dna to create as ssdna template at the replication fork. Rna primers must be removed to complete dna replication.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related textbook solutions

Molecular Cell Biology
8 Edition,
Lodish
ISBN: 9781464183393
Biology: Science for Life with Physiology
6 Edition,
Belk
ISBN: 9780134555430
Biology
2nd Edition, 2013
Openstax
ISBN: 9781947172517
Concepts of Biology
OER Edition,
Openstax
ISBN: 9781938168116
Essentials of Biology
5 Edition,
Mader
ISBN: 9781259660269

Related Documents

BIO372H5 Lecture Notes - Lecture 9: Mobile Genetic Elements, Dna Replication, Transposable Element
hassan_rizvi96
BIO372H5 Lecture Notes - Lecture 3: Dna Polymerase Iii Holoenzyme, Enzyme, Exonuclease
hassan_rizvi96
BIO372H5 Lecture Notes - Lecture 6: Okazaki Fragments, Semiconservative Replication, Deoxyribonuclease
skykoala223

Related Questions

Match the phrase its definition

DNA replication is semi-conservative

[ Choose ] place in which the two strands of DNA are being unwound and replicated the side of the DNA double helix that is synthesized continuously replication proceeds from the origin of replication in both directions simultaneously the side of the DNA helix that is synthesized using Okazaki fragments during DNA replication one strand is derived from the original DNA and the other strand is newly synthesized in the paired chains of the DNA helix adenine always binds thymine, and guanine always binds cytosine the two chains in the double helix are oriented in opposite directions

DNA replication is bidirectional

[ Choose ] place in which the two strands of DNA are being unwound and replicated the side of the DNA double helix that is synthesized continuously replication proceeds from the origin of replication in both directions simultaneously the side of the DNA helix that is synthesized using Okazaki fragments during DNA replication one strand is derived from the original DNA and the other strand is newly synthesized in the paired chains of the DNA helix adenine always binds thymine, and guanine always binds cytosine the two chains in the double helix are oriented in opposite directions

replication fork

[ Choose ] place in which the two strands of DNA are being unwound and replicated the side of the DNA double helix that is synthesized continuously replication proceeds from the origin of replication in both directions simultaneously the side of the DNA helix that is synthesized using Okazaki fragments during DNA replication one strand is derived from the original DNA and the other strand is newly synthesized in the paired chains of the DNA helix adenine always binds thymine, and guanine always binds cytosine the two chains in the double helix are oriented in opposite directions

leading strand

[ Choose ] place in which the two strands of DNA are being unwound and replicated the side of the DNA double helix that is synthesized continuously replication proceeds from the origin of replication in both directions simultaneously the side of the DNA helix that is synthesized using Okazaki fragments during DNA replication one strand is derived from the original DNA and the other strand is newly synthesized in the paired chains of the DNA helix adenine always binds thymine, and guanine always binds cytosine the two chains in the double helix are oriented in opposite directions

lagging strand

[ Choose ] place in which the two strands of DNA are being unwound and replicated the side of the DNA double helix that is synthesized continuously replication proceeds from the origin of replication in both directions simultaneously the side of the DNA helix that is synthesized using Okazaki fragments during DNA replication one strand is derived from the original DNA and the other strand is newly synthesized in the paired chains of the DNA helix adenine always binds thymine, and guanine always binds cytosine the two chains in the double helix are oriented in opposite directions

DNA is antiparallel

[ Choose ] place in which the two strands of DNA are being unwound and replicated the side of the DNA double helix that is synthesized continuously replication proceeds from the origin of replication in both directions simultaneously the side of the DNA helix that is synthesized using Okazaki fragments during DNA replication one strand is derived from the original DNA and the other strand is newly synthesized in the paired chains of the DNA helix adenine always binds thymine, and guanine always binds cytosine the two chains in the double helix are oriented in opposite directions

DNA is complementary

[ Choose ] place in which the two strands of DNA are being unwound and replicated the side of the DNA double helix that is synthesized continuously replication proceeds from the origin of replication in both directions simultaneously the side of the DNA helix that is synthesized using Okazaki fragments during DNA replication one strand is derived from the original DNA and the other strand is newly synthesized in the paired chains of the DNA helix adenine always binds thymine, and guanine always binds cytosine the two chains in the double helix are oriented in opposite directions

DNA synthesis: leading vs lagging strands during replication, Okazaki fragments, 5’ to 3’ direction vs 3’ to 5’ direction, meaning of ‘antiparallel’, role of DNA ligase, role of DNA polymerase

Transcription/translation, RNA/DNA, template strand vs non-template strand, introns vs exons

Relationship of DNA mutation to cancer.

Relationship of RNA to DNA, and RNA to proteins?

Meaning of semiconservative replication.

Codons (triplets) are part of what molecule?

Codons vs anticodons.

Functions of the different types of RNA.

Ribosome structure (including the E site, P site and A site).

1) What is the difference between the leading strand and the lagging strand in DNA replication? Place the following steps of DNA replication in right chronological order.

1. Primase adds an ribonucleotide primer
2. Initiator proteins bind the origin of replication and helicase breaks hydrogen bonds between complementary bases of double stranded template DNA
3. DNA polymerase III extends by complementary base pairing deoxyribonucleotides to the template strands
4. Ligase phosphodiester bonds the nucleotides to connect okazaki fragments nad connect fragments at the origin and where the "crotches or forks" collide
5. DNA polymerase I removes RNA primer and replaces with Deoxyribonucleotides


2) Given the template DNA and primer sequences below, what bases will be added to the primer as DNA replication proceeds? (The bases should appear in the order that they will be added)

5'-GTCGTCTCCCTTTTTAAAAACCCCCCC-3'
5'-UUUUUAAAAAGGG-3'


3) Given the DNA sequence below, what could be a primer sequence used in its replication?
5'-CGTACGGCCCCAAAATTGCTACTCTGCTG-3'