BIO206H5 Lecture Notes - Lecture 11: Kary Mullis, We Wanna, Chromosome
Document Summary
Cells have a remarkable ability to synthesize dna, and many other biological molecules. Chemists couldn"t make nucleotide sequences that were more than 20 or 30 nucleotides long, so they needed a method to be able chemically synthesize a speci c segment of dna from a chromosome in a pro or eu cell. The initial polymerase chain reaction was developed in 1983. This tells us that this invention completely revolutionized how we deal with dna. So this protocol has now become a back bone in every laboratory that does this work. In a chain reaction, positive feedback leads to a self amplifying step. Meaning we started with one thing, and ended with 5. We wanna be able to accurately replicate a fragment of dna in vitro. 1. dnaturation: dna molucels, double helix, are torn apart. 2. priming: identi es where on this large molecule of dna is the speci c part that we"re interested in.
