CHM110H5 Lecture Notes - Lecture 13: Manganese, Dependent And Independent Variables, Metal

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In this lab, 5 metal ions, mn+2, fe+3, co+2, ni+2, and cu+2, and an unknown substance containing 2 metal ions will be dropped on a piece of chromatography paper. The purpose of this experiment is to determine the rf values, or retention factor, of several cations and use them to determine the contents of an unknown substance using the thin paper chromatography methodological approach. The thin paper methodological approach involves a piece of chromatography paper, where a solvent bonds with different sample drops, and climbs the chromatography paper by capillary action. There are two phases of chromatography, the stationary phase, and the mobile phase. The stationary phase is the sample drops of 5 different cations, and an unknown substance, placed on the chromatography paper. The mobile phase is the solvent in which the stationary phase is experiment is a 9:1 ratio mixture of acetone, (ch ) co, and hydrochloric acid, hcl.

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