CHM361H5 Lecture Notes - Lecture 18: Pepsin, Subtypes Of Hiv, Acid Dissociation Constant
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One has a high low pka and the other has a high pka. The pkas are different because they are in an environment that has proteins of different charges, electrochemical properties and so this will change the pka as you are found as protonated or deprotonated. ** the pka given on test is based on bulk solutions which is different than the environment with proteins. The oh can bind to the aspartates in the active site. The c-terminal of the carbon that has the oh group. Cannot become a leaving group and so will bind into the pocket that oh will end up occupying. There is a c-h bond and thus no leaving group. This is different than the serine protease which are in the gut (low ph) There is still activity at high or low ph. Most proteins are denatured at very high ph or low ph. This depends on the enzyme and their function.
