PSY355H5 Lecture Notes - Lecture 6: Thymidine, Neomycin, Zygosity
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If no tk on gene = no effect. Cellular level, not allelic: es cell + null, es. Cell + es cell, null + null (hom) Es cell + null (het) x es cell + null (het) Es cell + es cell , es cell + null , es. Cell + null , wild type: how do you know which are homozygous, check for functional protein or check for neomycin sequence signal. Behavioural tests: to solve the task mutant is forced to use visual stimuli -> altered visual functioning -> altered visual physiology, gene expression, etc. Inducible systems can limit timing of genetic manipulation on specific period (meaning can induce gene to turn off and on at specific times) Homologous recombination in 129 type (mouse strain) es cells. Problem with 129 type: suffers from different issues even without mutations, ma(cid:374)(cid:455) do(cid:374)(cid:859)t ha(cid:448)e (cid:272)o(cid:396)pus (cid:272)allosu(cid:373) Find strain with pure genetic bgs (that works)
