BIOB10H3 Lecture Notes - Lecture 3: Transmissible Spongiform Encephalopathy, Prnp, Golgi Apparatus
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Unit 2 Organic Chemistry
Organic compounds vs. Inorganic compounds
What makes a compound organic? Inorganic?
Functional groups (phosphate groups, amino groups, carboxyl groups)
Know the structure of each of these groups, and which organic compounds they are found in. You can draw them for your own use.
Hydrolysis
What is it? What is it used for?
Dehydration synthesis
What is it? What is it used for?
Carbohydrates (Monosaccharides, Disaccharides, Polysaccharides and examples)
Know the process of joining two monosaccharides together to create a disaccharide.
Be able to give examples of several monosaccharides, disaccharides and polysaccharides.
Monosaccharide examples (3) | Disaccharide examples (2) | Complex carbohydrates (3) |
Lipids (Saturated vs. Unsaturated; Phospholipids, Steroids)
Define a saturated fatty acid.
Define an unsaturated fatty acid. Draw if it helps.
What process is used to put the fatty acid chain on a glycerol molecule?
Hydrophilic vs. hydrophobic
Define hydrophilic.
What part of the phospholipid is hydrophilic?
Define hydrophobic.
What part of the phospholipid is hydrophobic?
Proteins, types and functions
What is a protein composed of, and what does it do?
Amino acids
Name the three parts of the amino acid that come off the common Carbon.
Identify each of the three parts. (Amino Acid ID game)
Peptide Bonds
How are they formed?
What two parts of each amino acid do they join together?
Enzymes - define.
Levels of Protein structure
Complete the chart below.
Protein structure level | definition |
Primary | |
Secondary | |
Tertiary | |
Quarternary |
Protein Denaturation
What is it and how does it happen?
Define what a Solute is.
Define what a solvent is
Nucleic acids (DNA, RNA, ATP)
Nucleotide
Name its three parts and be able to identify each of them on a diagram. (Nucleotide ID game)
What are the two possible sugars used in a nucleotide?
Complete the Table below.
DNA | RNA | ATP | |
What sugar does it have? | |||
What nitrogen bases does it have? | |||
Provide the pairings of the nitrogen bases of each nucleic acid | N/A | ||
What is its structure? |
BACKGROUND
The amino acid sequence for a protein that is associated with the onset of Huntington's Disease in humans is 582 amino acids long and is provided below. This disease is autosomal dominant and results in a prolonged degeneration of nerve cells in the brain.
INSTRUCTIONS
1. For this assignment, use any method you determine most feasible (i.e. programming in MATlab, R script, Crystal Ball, Witchcraft/Hocus Pocus, etc.) to decipher the amino acid composition of this protein and answer the 15 questions associated with this protein and its associated amino acid structure.
2. You will be calculating proportions (percentages - %) for this assignment. Calculate all proportions to 1 decimal place and make sure you have rounded up or down correctly. If unsure, use a spreadsheet program such as Excel to display your proportional calculations to 1 decimal place.
3. Spelling matters (Incorrect spelling will be graded as such - incorrect).
5. Download/copy the sequence and review the questions so you can double check your numbers before proceeding through the homework.
Huntington Disease Associated Protein Amino Acid Sequence
The first amino acid below ('m') represents the amino (NH3) terminus of this protein. The last amino acid in this sequence represents the carboxy (COOH) terminus and is the very last 'c' at the bottom of this protein sequence structure. The amino acids are numbered individually across the rows (left to right) beginning from the amino terminus (#1) to the carboxy terminus (#582).
mvskreniat | hhlyqawdpv | pslspattga | lisheklllq | inperelgsm | syklgqvsih |
svwlgnsitp | lreeewdeee | eeeadapaps | spptspvnsr | khragvdihs | csqfllelys |
rwilpsssar | rtpailisev | vrsllvvsdl | fternqfelm | yvtltelrrv | hpsedeilaq |
ylvpatckaa | avlgmdkava | epvsrllest | lrsshlpsrv | galhgilyvl | ecdllddtak |
qlipvisdyl | lsnlkgiahc | vnihsqqhvl | vmcatafyli | enypldvgpe | fsasiiqmcg |
vmlsgseest | psiiyhcalr | glerlllseq | lsrldaeslv | klsvdrvnvh | sphramaalg |
lmltcmytgk | ekvspgrtsd | pnpaapdses | vivamervsv | lfdrirkgfp | cearvvaril |
pqflddffpp | qdimnkvige | flsnqqpypq | fmatvvykvf | qtlhstgqss | mvrdwvmlsl |
snftqrapva | matwslscff | vsastspwva | ailphvisrm | gkleqvdvnl | fclvatdfyr |
hqieeeldrr | afqsvlevva | apgspyhrll | tclrnvhkvt | tc | CARBOXY TERMINUS |
I posted this before with no replies! It's urgent.
I need this decoded because I don't have any access to any coding methods in order to answer the following questions:
a.) a.) What is the name of the least abundant amino acid in this protein?
b.) What proportion of the total amino acids in the protein does this individual amino acid represent?
c.) What is the proportion of essential amino acids included in this protein sequence?
d.)The amino acid sequence from residues 73-85 is represented by the amino acids EEEWDEEEEEEAD, how would you generally classify this small region of the protein? Choose from: neutral, hydrophilic, hydrophobic, negatively charged, positively charged
e.)Two amino acids are present in the same proportion in this protein, which two are those?
f.)Which class of amino acids is the most abundant in this protein?
g.) What amino acid(s) is/are present at locations 572 and 582 in the Huntington Disease protein (Full Amino Acid Name(s)).
h.)What type of structure could form between these amino acids that could help stabilize this protein's structure?
i.) Which class of amino acids is least abundant in this protein?
j.)Summed together, how many hydrophilic amino acid residues are present in this protein?