BIOB11H3 Lecture Notes - Lecture 10: Okazaki Fragments, Dna Replication, Flap Structure-Specific Endonuclease 1
15 views5 pages
Document Summary
Eukaryotic cells: replicons small portion of dna replicated by eukaryotes, has own ori there are 10,000- 100,000 in human cells: dna synth initiation in replicons highly. Pol regulated: replication in higher eukaryotes can depend. Pol on: positions of nucleosomes, types of histone modifications, dna methylation. Has 3"-5" exonuclease activity (synthesis of lagging strand) Continues synthesis when it reaches a previous okazaki fragment. Flap of rna sticks out and is removed by fen-1 endonuclease. Dna spooled through replication machinery: nucleosomes are displaced during replication but reassemble quickly. A/g come off sugar backbone due to normal metabolism. Initiated by a dna glycosylase that recognizes the altered geometry and wrong nucleotide. Removes base by cleaving glycosidic bond attaching base to deoxyribose sugar. Transcription coupled pathway repairs actively transcribed dna (high priority) Global genomic pathway repairs other errors and is slower. 2 strands are separated by tfiih subunits with helicase activity (xpb and xpd)
Get access
Grade+
$40 USD/m
Billed monthly
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
10 Verified Answers
Class+
$30 USD/m
Billed monthly
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
7 Verified Answers
Related Documents
Related Questions
1. | In addition to identifying the genetic material, the experiments of Avery, MacLeod, and McCarty with different strains of Streptococcus pneumoniae demonstrated that | ||||||||||
|
2. | In order to show that DNA in cell extracts is responsible for genetic transformation in Streptococcus pneumoniae, important corroborating evidence should indicate that _______ also destroy transforming activity. | ||||||||||
|
3. | Based on what you have learned about the experiments conducted by Griffith and Avery and colleagues with bacteria, which of the following would result in transformation of living R cells? | ||||||||||
|
4. | A-T base pairs in a DNA double helix | ||||||||||
|
5. | If 23 percent of the bases in a sample of double-stranded DNA are adenine, what percentage of the bases are uracil? | ||||||||||
|
6. | The uniform diameter of the DNA structure provides evidence for | ||||||||||
|
7. | If a sequence of one strand of DNA is 5â²-TGACTATC-3â², what is the complementary strand? | ||||||||||
|
8. | What structural aspect of the DNA facilitates dissociation of the two DNA strands for replication? | ||||||||||
|
9. | If the MeselsonâStahl density gradient experiment had resulted in two bands of DNA molecules after only one round of replication, one containing only 15N and the second only 14N, this result would have indicated that replication was | ||||||||||
|
10. | The nucleoside analogue acyclovir, which is used to treat herpes simplex virus (HSV) infections, lacks a 3â² hydroxyl group (âOH). Predict what will happen if the host cell DNA polymerase incorporates a molecule of acyclovir into an elongating strand of HSV DNA. | ||||||||||
|
11. | Which of the following does not demonstrate the stability of the DNA double helix? | ||||||||||
|
12. | What effect would a primase inhibitor have on DNA replication? | ||||||||||
|
13. | To replicate their DNA in a timely manner, most eukaryotic chromosomes | ||||||||||
|
14. | Which statement about DNA replication is false? | ||||||||||
|
15. | In many eukaryotes, there are repetitive sequences called telomeres at the ends of chromosomes. After successive rounds of DNA replication, the _______ strand becomes shorter. In some cells, an enzyme called _______ repairs the shortened strand. | ||||||||||
|
16. | A researcher studies normal human fibroblast cells. They can be maintained in culture but die off after about 30 cell generations. Unexpectedly, a colony of cells continues to survive and divide past 30 generations. Which scenario is most likely true for these cells? | ||||||||||
|
17. | If DNA polymerase III introduces an incorrect nucleotide, what is the first corrective action made by the DNA repair system? | ||||||||||
|
18. | Choose the correct order of the following four events in the excision repair of DNA: (1) Base-paired DNA is made complementary to the template. (2) Damaged bases are recognized. (3) DNA ligase seals the new strand to existing DNA. (4) Part of a single strand is excised. | ||||||||||
|
19. | Six complete cycles of PCR should result in a _______-fold increase in the amount of DNA. | ||||||||||
|
20. | When double-stranded DNA is heated to temperatures above 90°C, it denatures. Denaturation is a process that | ||||||||||
|