BIOC14H3 Lecture Notes - Lecture 5: Integrase, Olfactory Bulb, Recognition Sequence
11 Mar 2015
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1. containing multiple copies of randomly integrated dna. Capecchi: when multiple copies of dna introduced into nuclei, they are integrated into random sites within mouse genome that are following each other) due to homologous recomb. Transgenic littermates identified by pcr analysis of tail samples performed at 3 weeks old. Which offspring has transgene product: application of pronuclei injection methods (over)express wt genes (over)express mutant genes (over)express reporter genes (fluo genes we can label: gfp, rfp, Lacz: cell type specific overexpression of a transgene. Need to link to a cell type specific promoter sequence to the gene of interest -> inject to directly express gene in specific cell type. Capecchi: concatemers and what implications they have, i. e. how they arrive at homologous recombination method: concatemer. Highly ordered -> added dna sequences are not randomly oriented within the concatemer (same direction of head-to-tail) random ligation process, but either by. Reasoned that concatemers could not be generated by a.
