BIOC32H3 Lecture Notes - Lecture 2: Biophysics, Electrochemical Gradient, Tandem Pore Domain Potassium Channel
7 Sep 2017
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Explanations comes from understandings and research in various fields. Physiologists --> explain behaviour using rules of cellular response. Biophysicists --> how cellular response govenerned by physical chemistry and electricity. Use kinetic models and rules of thermodynamics to research hypothesis. Julius berstein --> neurobiologist and biophysicist and came up with. Cells have electrolytic interior surrounded by membrane impermeable to ions. There is a resting difference of potential across membrane (resting. The high intracellular k+ concentration and high extracellular cl- concentration create a gradient. Exceptions include water and small ions (k+ and cl-) Use electrophysiology to measure membrane potential difference of living cells (figure 5. 30) Voltmeter measures difference between grounding (extracellular) and recording (intracellular) electrode. Difference between extracellular and intracellular space is the potential difference. Energy to drive movement between the two compartments. Exceptions are red blood cells and spinal cord neurons. Unless the cell is an excitable cell, cells will remain in rmp.
