BIOC14H3 Lecture Notes - Lecture 5: Homologous Recombination, Diphtheria Toxin, Neomycin
24 Apr 2013
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Bioc14winter2013 lecture 5: mouse genetic engineering and genetic dissection of neural circuits (article 1 and article 2) Generating transgenic mice (2 approaches: dna microinjections into single cell embryos, produce transgenic mice containing multiple copies of randomly integrated dna. Dna microinjection into single cell embryos: 1. Fertilized once-cell eggs for dna microinjection are obtained from superovulating female mice that have been mated to stud made: 2. Purified experimental dna is directly injected into the male pronucleus of a fertilized mouse egg using a microneedle male pronucleus is the nucleus of the sperm from the male (the sperm is in the egg, but transfer of. Following microinjection, 20-30 eggs are impanted into the oviduct of pseudopregnant surrogate female (recently mated to vasectomized male) female is bread by infertile male, such that all symptoms of pregnancy occur (and egg without embryo develops: 4.
