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BIOC23H3 (23)
Lecture

BIOC23: Lec 5 - Partition chromatography.doc

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Department
Biological Sciences
Course
BIOC23H3
Professor
Rongmin Zhao
Semester
Winter

Description
Lec 5 Partition ChromatographyTypes of partition chromatography1 Paperostationary water supported by celluloseomobile solvent that moves by capillary actionoresolution not as good as TLC2 Thin Layerostationary water bound to solid coated onto glassplastic plateomobile solvent that moves by capillary actionPartition chromatographyanalytical usually identify compound determine puritymonitor purificationPaper chromatographySolid support cellulose glucose polymer hydrophilicStationary phase Water polar absorbed onto paperMobile phase Butanol nonpolarCH3CooHH2O free AA are more water solubleTLCSolid support glass aluminum or plastic on top of this is thin layer of adsorbentStationary phase solvent used to prepare
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