CHMB16H3 Lecture Notes - Petroleum Ether, Theobromine, Reliability (Statistics)

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14 Oct 2012
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Chmb16fall2012 lecture 1: introduction to analytical chemistry (chapter 0) Definitions: qualitative analysis figuring out what is in your sample, quantitative analysis figuring out amount of x in your sample, analyte = targeted molecule. Selecting a method: when selecting a method of research of the analyte, factors such as time, efficiency and cost must be taken into consideration. Obtaining the sample: there are 2 types of samples: heterogeneous and homogeneous (pure, separation techniques must be performed on heterogenous samples in order to obtain the analyte. Eliminating the interferences: the matrix, calibrating and measuring concentration, calculating results, evaluating results by estimating their reliability (statistics) Classifying chemicals: reagent grade(minimum standards set by acs, primary standard grade (purity set by nist, special purpose reagent chemicals (sigma-aldrich, emd chemicals, etc) Example: how much caffeine is in chocolate: first, pestle and mortar is used to grind up the chocolate. In chocolate there is caffeine and theobromine which is very structurally similar to caffeine.

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