ANT100Y1 Lecture Notes - Lecture 7: Microtubule Organizing Center, Intermediate Filament, Fluorescence Microscope
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The cytoskeleton provides structural support: rbc poked with laser tweezers returns to its original shape due to structural support provided by cytoskeleton. It withstands tears and allows them to bring back to original shape. 3 types of filaments form the cytoskeleton: microfilaments: actin proteins. Form long filaments made of protofilaments that are twisted around each other. Has rope-like qualities: microtubules: made of turbulent proteins. Individual protofilaments allow structure to be organized to microtubule organised centre. Immunoflorescence: technique used to determine the location of proteins within cell. Light microscopy has resolution limit due to diffraction based on wavelength of light (250 nm) Dynamic structure: electron microscope is not useful for this: for each cell motility/crawling the actin filaments must rapidly assemble and disassemble at the leading edge. Eg. a neutrophil chasing clump of bacteria. How are these polymers constructed: need to be strong so that they can have support functions within cell.
