BIO130H1 Lecture Notes - Lecture 14: Integral Membrane Protein, Lipid Bilayer, X-Ray Crystallography

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12 Jul 2017
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BIO130H1 Full Course Notes
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Fluid mosaic model: lipid molecules arranged in regular antiparallel bilayer. They are associated with the lipid bilayer in different ways: integral membrane proteins: pass through lipid bilayer, helical structure (common structure for most membrane proteins) Can be single or multipass: peripheral membrane proteins: either associated with lipids or associated with other proteins. Some lipids bind proteins and localize to membrane: gpi or lipid-anchored. Amphipathic (have polar and nonpolar domains/hydrophilic and hydrophobic domains) Hydrophilic domains: aqueous, amino acid side chains are polar (loops or tails that stick out on either side of membrane) Hydrophobic membrane-spanning domain: amino acid side chains are nonpolar, interact with lipid tails, interact with hydrophobic tails of phospholipids. Multipass: multiple helices hydrophilic amino acid side chains hydrophobic amino acid side chains. Special hydrophobic helix that goes through membrane, helical structure is same. Side chains point out all around helix into membrane, interact on all sides with phospholipid tails. 3d structures, phospholipids all the way around, hydrophobic.

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