BIO130H1 Lecture Notes - Lecture 2: Condensation Reaction, Hemoglobin, Proteome
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BIO130H1 Full Course Notes
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Readings: p77-79, 393-397, 33-42, 50-61 skim: 62-63 (fig. 2. 41) 70-72 (up to protein microarrays) Nucleic acids are the genetic material in a cell is organism"s blueprints: dna = deoxyribose nucleic acid, rna = ribonucleic acid. 3 type of bases: purines: adenine, guanine (double nitro-base ring, pyrimidine: thymine, uracil (singe nitro-base ring) Dna is made with star stuff except for phosphorus, it is made during an explosion of a star. Dna vs rna: dna: deoxyribose, gcat, rna: ribose, gcau (uracil, rna has reactive oh group located on 2" carbon. Dna can be defined as 2 molecules (2 strands), however some say dna is 1 molecule composed of 2 strands. Heating dna and high ph can break the hydrogen bonds and split dna strands. Slowly cooling or lowering ph back to 7 can re-anneal the dna strands. (reversible process: melting temperature = temperature when dna is unzipped, separating dna is a reversible process.