BIO130H1 Lecture 8: Lecture 8 Part 1

• School: UTSG
• Department: Biology
• Course: BIO130H1
• Professor: Jane Mitchell

Document Summary

Eukaryotic genetics: most genes code for mrna, majority of rna created is not mrna. Differences from prokaryotic transcription: contains introns and exons (segments of dna) Exons: transcribed, may or may not be translated: dna primary rna transcript manipulation of rna mrna. Transcription occurs in nucleus: use general transcription factors (group of proteins) instead of sigma factor, general transcription factors help position rna polymerase at promoter, deal with chromosomes. Rna polymerases (rnaps: rna polymerase i: catalyzes rrna transcription, rna polymerase ii: all protein-coding genes, mrna, rna polymerase ii contains carboxy terminal domain (ctd) needed for transcription, rna polymerase iii: trna. Notation: upstream negative value, nucleotide points before transcription initiation, downstream positive value, nucleotide points after transcription initiation. Transcription starts at +1 value, there is no 0 value. Promoter site: group of nucleotides that help position rnapii for transcription. Tata box (promoter site: helps position rna polymerase ii at +1 site to start transcription.