BIO130H1 Lecture Notes - Lecture 10: Intermediate Filament, Cytoskeleton, Microvillus
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BIO130H1 Full Course Notes
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Positions organelles so they are next to correct compartments. Cell division: cytoskeleton structure needs to change to form spindles to divide. Three types of filaments form the cytoskeleton (1) microfilaments. Made of 2 proto-filaments that are twisted around each other. Organized in certain regions (2) intermediate filaments. Generally organized to 1 point on nucleus and radiate out to pm. Recall with pulse-chase (every protein labelled w/i certain window was labelled didn"t matter what type of. Technique to determine location of specific protein in cell protein) This experiment only labels very specific protein of interest (ex: where is actin?) A primary antibody is used to bind specifically to the protein of interest. A secondary antibody binds to primary antibody and is covalently tagged w/ fluorescent molecule. Fluorescence microscope used to excite fluorescent molecule and visualise light emitted. Light microscope has resolution limit: because of diffraction, based on wavelength of light (~250nm, microtubules have diameter of 25nm.
