BIO260H1 Lecture Notes - Lecture 9: Operon, Chromogenic, Long Term Ecological Research Network
Document Summary
07:17 ames did three things to test for mutagens by increasing chances of mutation. 07:35 1. introduced rfa mutation: increases membrane permeability. 08:00 3. concentration - mutation proportional to concentration of mutagen. 11:13 only 10% of chemicals he tested were mutagens(known carcinogens). 13:20 his- cells incubated with liver extracts and the chemical tested for certain amount of time to see breakdown products. 13:57 this increased from 10% to more than 90% 14:31 he would nd there was a halo around the middle. Too much mutations elsewhere and hence cannot live. At a lower concentration it is able to live and also able to revert mutation. 20:56 gene encodes for one enzyme from beadle and tadum. 22:03 dna transcribed to mrna which is translated to proteins. 25:12 regulation could be at translation or transcription level. 26:40 there could be transacting factors or the protein itself driving the transcription/translation of itself.
