BCH210H1 Lecture Notes - Lecture 7: Multiple Cloning Site, Dna Ligase, Recombinant Dna
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BCH210H1 Full Course Notes
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Ch 2 (q 1(abc), 2(abc), 3, 4, 19b) Lecture 7/12 - intro to proteins and protein structure. Genetic engineering has revolutionized the study of protein structure/function. Allows us to ask sophisticated questions about the role of any one aa in a protein. Replace one and investigate what happens --> whether you completely remove the properties or keep a property and just change the amino acid. Study naturally-occurring mutations found in nature, such as sickle cell anemia. There are some cases when you cannot extract protein from cells (minute traces) Combo of genetic techniques (site-directed mutagenesis) and expression in cells. Bacteria usually uses e. coli; possible for mammalian cells also. The proteins and their mutants can be obtained in quantities sufficient for research. Can study in the cell itself as well. Clone gene of interest from genome or cdna (complimentary) from mrna --> from pcr. Humans have introns which is why you take the mrna.
