BCH210H1 Lecture Notes - Lecture 8: Peptide, Nucleophile, Lysine

• School: UTSG
• Department: Biochemistry
• Course: BCH210H1
• Professor: Stavroula Andreopoulos

Document Summary

The sample is added th the wells, and we apply an electric charge to the smaller proteins are at the bottom, larger ones are at the top. Coomasie blud is a dye that loves proteins. The bands in the lanes represent different polypeptides, they are all different sizes. To see if this is our protein, we add a tag like an epitome which is 6-8 residues in length that we insert in the protein. This epitome will be recognized by a specific antibody. After the proteins are on the sheet of apper, we incubate them with the antibody. The flow through is the stuff that does(cid:374) t (cid:271)i(cid:374)d to the (cid:272)olu(cid:373)(cid:374). If we have lots of protein, the enzyme makes the reaction last 5 seconds. If we have low proteins, the enzyme will still get the desired results, it will just take longer. This is 1 polypeptide, but its made up of 2 domains.