BCH210H1 Lecture Notes - Lecture 8: Wild Type, Alanine, Hydrophile

• School: UTSG
• Department: Biochemistry
• Course: BCH210H1
• Professor: Stavroula Andreopoulos

Document Summary

Sds gel electrophoresis: sds-page: sodium dodecyl sulfate. Polyacrylamide gel electrophoresis: sds: denaturing detergent, dissolves membranes and solubilizes proteins, polyacrylamide: gel matric to separate proteins. Powerful technique to separate polypeptides based on their molecular weight. Sensitive to analyze g amounts of protein (10-15 g), for analytical purposes, not purifying proteins. Sds (like urea) unfolds proteins and denature them, and as a consequence the proteins are inactive, it can also membranes and solubilize proteins in the membrane used for studying membrane proteins. Polyacrylamide is a matrix made of acrylamide that forms a gel used to separate the proteins. Sds has a hydrophobic region (dodecyl, or lauryl) alkyl chain and a charged hydrophilic sulfate region, with a neutralizing ion in the solution. Separating sds-protein complex, and since sds is negatively charge, it"ll move towards the positive anode: gel is a matrix so small proteins can migrate faster while large proteins remain at the top.