BIO120H1 Lecture Notes - Lecture 16: Electrophoretic Mobility Shift Assay, Polyacrylamide Gel Electrophoresis, Sequence Motif
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BIO120H1 Full Course Notes
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Last lecture: taken a look at the interplay b/w dna & its organization & its transcription to rna & the regulation of those processes. When we did that, started to talk about the components of genetic switches & talked about the role of sequence specific transcription factors/gene regulatory proteins & their interaction with short segments or motifs of dna. Imagine that that promoter is actually chalk-block with different motifs, one that is important for, for example, driving expression in guard cells, another chunk of that promoter may be important for driving expression, for example, xylem cells. Way we can look at the interplay b/w a protein & a dna segment is done very nicely in vitro (outside of the cell) using electrophoresis. Have indentified target sequence, now we want to ask can a specific protein interact with those sequences. Just imagine that we"ve already identified a protein that can interact with this target motif.
