BIO130H1 Lecture 5: Lecture 5 Hour 2
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BIO130H1 Full Course Notes
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Document Summary
Follows the same steps as circular dna replication: only differences include, double helix unwinding process, telomere issue at ends of replication strands. Problem at the ends of linear eukaryotic dna: occurs on lagging strands, sometimes the space at ends is too small, dna primase cannot attach itself or. If dna primase does attach itself, the rna primer will be removed: rna primer is removed as there is no space for dna polymerase to continue strand. Telomere solution: repetitive dna sequence on 3" end of parental lagging strands, added by protein called telomerase, adding on 3" of lagging strand as is it reached last during lagging replication. Telomerase contains an rna template that helps to elongate the dna strand (telomere: works in 5"-3". This process of creating dna strand from rna template called reverse transcriptase. Telomeres are usually g-rich, meaning rna template is c-rich. Telomere also helps protect dna from proteins that splice single stranded dna.
