CSB327H1 Lecture Notes - Lecture 18: Elastin, Fibrillin, Basal Lamina
28 Dec 2012
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Document Summary
Structure of artery: endothelium basal lamina elastin smooth muscle elastic connective tissue epithelium. In stretched state, the water is organized (low entropy) In the recoiled or relax state, the water is disorganized (high entropy) Coacervation of tropoelastin (phase separation and aggregation of te: endothermic, entropy driven process, caused by multiple interactions of individual hydrophobic domains. Change in temperature, ph, hydration, or salts (ionic strength) will force te out of solution. Increasing the temperature will force te out of solution. Decreasing the temperature makes te soluble in aqueous solutions. Hydrated te decreases the temperature at which coacervation can occur. Interacts with fibrillin: rich in hydrophobic residues (pro, gly, ala, val, since there are no internal met residues (signal peptide was cleaved), elastin can be purified by digesting connective tissue with cnbr. Molecular organization of tropoelastin: hydrophobic domains (beta sheets) important for assembly and elasticity. Large hydrophobic side chains above and below the plane of the beta sheet.
