Lecture 24

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Cell and Systems Biology
Mounir Abou Haidar

Rhabdoviruses Vesicular Stomatitis Virus VSV Rabies Virus RabiesLike Virus Plant rhabdoviruses Ungrouped rhabdovirusesRecall he went over the genes PGLetc genetics how the different pieces of sub genomes are made and replication last timeRecall point 2 the positioning of the gene on the genome determines the control of transcription The closer a gene is to the 3 the greater the transcription for the gene the farther away it is from 3ie closer to 5the less transcription there is of the gene Thus subgenomic formation is higher at the 3 end than the 5 end Pg 151VSV replication at 155 minPhosphatidylserine receptor is the molecule on the surface of cells to which the virus attaches via its G glycoprotein G is the surface envelope protein After adsorption of the G protein receptor mediated endocytosis follows The vesicle then fuses with the endosome followed by uncoating The RNA including the RdRp RNA dependent RNA Polymerase bound to it is released into the cell Recall that a few copies of the RdRp are needed for transcription of the genome to make different subgenomic RNA The different subgenomes of all the genes on the virus have a 5 cap and 3 polyA tail The polyA tail is produced by skidding or stuttering of the RdRp on polyU stretches at the end of each gene on theRNA This polyU sequence is a transcription terminator responsible for producing the polyA tail These separate mRNAs are translated into proteins This is the transcriptive mode of RdRp Recall point 2 see above in green After uncoating transcription follows since the polymerase is bound to the RNAIn the replicative mode of RdRp the polymerase takes the RNA and copies it faithfully from the 3 to 5 end to make a full RNA for the entire genome At this time all intergenic regions are ignored the intergenic regions have the stop signal during the transcriptive mode of the RdRp We dont know why this polymerase acts this way The RNA has no cap at 5 it has no polyA tail at the 3 end This RNA is not translatable This is used to faithfully copy and make more RNA via the RdRp Nothing is added or deleted in the replicative mode Proteins are made by ribosomes in the cell from the mRNA made in the transcriptive mode The virus begins to assemble G protein is made by membrane bound ribosomes and is sent to the Golgi for glycosylation and then it is shipped to the cell membrane where it is anchored When the virus is completely assembled it buds and tears a piece of the cell membrane taking its own G protein with it in the envelope The virus is released These viruses are released by budding and are highly cytopathogenic have a strong effect on cells and kill them essentially
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