CSB351Y1 Lecture Notes - Polyadenylation, Plant Virus, Antibody
15 Feb 2012
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Rhabdoviruses: vesicular stomatitis virus (vsv), rabies virus, rabies-like virus, The closer a gene is to the 3", the greater the transcription for the gene; the farther away it is from 3" - i. e. closer to 5" - the less transcription there is of the gene. Thus, subgenomic formation is higher at the 3" end than the 5" end. Pg 151 - vsv replication at 1:55 min: phosphatidylserine receptor is the molecule on the surface of cells to which the virus attaches via its g glycoprotein. G is the surface envelope protein: after adsorption of the g protein, receptor mediated endocytosis follows. The vesicle then fuses with the endosome, followed by uncoating. The (-)rna, including the rdrp (rna dependent rna polymerase) bound to it, is released into the cell. Recall that a few copies of the rdrp are needed for transcription of the genome to make different sub-genomic rna.
