CSB353H1 Lecture Notes - Lecture 4: Chitin, Defensin, Symbiosis
28 Apr 2016
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Document Summary
Transcriptional activation of specific defense genes is important part of inducible defense. Pr proteins induced in tissue infected by pathogens as well as systemically. Indirectly releasing breakdown products from fungal cell walls, which act as signal molecules to activate plant defenses. Many pr genes are induced not only by pathogen infection, but also by sa treatment. Sa often observed after inoculation with biotrophic pathogens in plants. Pr gene expression is used as a marker for pathogen resistance activation and sar accumulation: infect plants with incompatible pathogen that induces a response look for mutant that doesn"t induce response find which gene was mutated. Pr gene expression is used to screen pathogen resistant mutants: no infection of pathogen look for plant that already has activated defense constitutive expresser of. Gus is reporter gene that shows expression of gene visually. If infect with pathogen, pr2 protein will be produced, and plant will be indigo colour due to gus expression.
