CSB328H1 Lecture Notes - Lecture 2: Limb Development, Fate Mapping, Morula
Document Summary
Opening up the top of the egg to make observations. Unsaturated fatty acids, protein, minerals, vitamins, cholesterol only found in the egg yoke. Studies for limb development: elegans, small egg (50um, easy to breed, cheap to feed (eat bacteria, 300 eggs in 2-3 weeks, transparent eggs and embryos. Cells divide and become smaller since egg is the same size. A lot of movement: external development. Lay eggs (outside mother: very rapid embryonic development (15 hours, very rapid life cycle, egg to adult worm (3 days, small adult worm size (1mm, physical manipulation (limited, cell ablations, highly developed genetics easy genetic manipulation. Pigmented (polarity even before fertilization takes place) and surrounded by gelatinous coat. Develop synchronously: hundred of eggs laid simultaneously, eggs and embryo not transparent (disadvantage, external development independent of mother, rapid embryonic development (4 days, adult frog size (large 10-13cm, maintenance is cheap and easy to breed.