CSB452H1 Lecture 2: Lecture 2 Summary

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Ttss in gram-negative bacteria is needle like with long pilus similar to flagella. hrp = hypersensitive reaction and pathogenecity genes. Exchangeable effector locus hrc gene cluster conserved effector locus. hrc gene cluster is conserved core operon with hrcl regulatory gene in pathogenicity island. virulent genes only present in virulent strains. Flanked by direct repeats or bordered by trna or mobile elements. hrcl is master tf: with signal, protease stop degrading hrcr. Identified 1 factor that cause hr in soybeans. grow p. syringae pv glycinea and prepare shredded gdna and transform gdna into e. coli colonies. Triparental mating: donor with psg6dna + helper e. coli + recipient with psg5 lacking avr gene. grow psg5 transconjugates and inoculate on plant leaf to assess hr. Ttse avr gene then recipient should cause hr. all ttse contain secretion signal + catalytic/binding domain with optional chaperone binding site.

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