HMB265H1 Lecture Notes - Lecture 11: Statistical Significance, Adenine, Association Mapping

• School: UTSG
• Department: Human Biology
• Course: HMB265H1
• Professor: Maria Papaconstantinou

Document Summary

Information from this lecture is not tested on the midterm: midterm notes posted on blackboard, some review on slides from today. Took ten years to discover - on chromosome 4. Technique used to identify was linkage mapping and pedigrees. Very large family was used for these mapping studies. Scientists found markers that were very closely related. 1993: scientists used positional cloning to pinpoint the huntington"s disease. Particular function for this gene is not entirely known. Ubiquitous throughout the body and not so similar to other proteins. Maps based on phenotypes do not cover enough of genome. Allows genes and mutations of interest to be identified more rapidly and easily. Determine physical location of large pieces of dna (large-insert clones) Used by collins for the human genome project. Make and sequence these segments then find the overlaps. This allows to create the entire segment - results in the contig. Take dna and break it up randomly into small fragments.