HMB265H1 Lecture Notes - Lecture 11: Zygosity, Open Reading Frame, Contig

• School: UTSG
• Department: Human Biology
• Course: HMB265H1
• Professor: Maria Papaconstantinou

Document Summary

Slide 9-11: hierarchical approach; first determine the physical location of large pieces and sequence these pieces. you need a physical map with large pieces of insert clones of dna and the(cid:374) you (cid:271)(cid:396)eak the(cid:373) up i(cid:374)to s(cid:373)alle(cid:396) f(cid:396)ag(cid:373)e(cid:374)ts/pie(cid:272)es. The(cid:374) you"(cid:396)e goi(cid:374)g to se(cid:395)ue(cid:374)(cid:272)e/(cid:272)o(cid:374)st(cid:396)u(cid:272)t this genome from the small pieces. These dna fragments are overlapped, and they look for identical places where all these segments overlap. All at once sequence whole genome in many small pieces. Looking for identical sequence in between the contigs. Hierarchical: generating an overlapping set of intermediate sized clones, and keeping a map of that. It took 2 years for the mapping of e-coli. The advantages allow sequencing of individual clones in different labs and each stretch of dna only needs to be sequenced once. This se(cid:395)ue(cid:374)(cid:272)i(cid:374)g is also i(cid:374)depe(cid:374)de(cid:374)t of ea(cid:272)h othe(cid:396) so e(cid:448)e(cid:374) if o(cid:374)e se(cid:395)ue(cid:374)(cid:272)e is (cid:449)(cid:396)o(cid:374)g, it (cid:449)o(cid:374)"t hi(cid:374)de(cid:396) the rest.