HMB265H1 Lecture Notes - Lecture 2: 5, Liposome, Endosome
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HMB265H1 Full Course Notes
Verified Note
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Document Summary
Adenovirus into the cell via various surfaces it needs to cross (epithelial; Explore how do adenoviruses get out of endosomes. Figure 1 - alpha sarcin assay hasn"t packaged protease or packaged only little amount so precursor proteins haven"t been cleaved) Comparing adenovirus wildtype type 5 vs ts1 (hyper stable because. Alpha sarcin is toxin - when into cell, cleaves ribosomal rna - so shuts down protein synthesis. Toxin cannot trigger its uptake, so endocytosed with adenovirus. Cannot get out of endosome; while in endoxome, not toxin becuase no access to rrna; see if toxin is released from endosome. Use s35 methionine - look for incorporation of radioactive methionine into newly made proteins. Ts1 - 39 means virions made at 39 degrees, the non. Ad5 can disrupt endosomal membrane, releasing alpha sarcin into cytoplasm; while ts1 does not do so efficiently; this says ts1 doesn"t effectively infect cells because it gets stuck in endosome.
