PSL350H1 Lecture 2: Lecture 2- Genetic Manipulations in Mice II.pdf
Document Summary
Lecture 2: genetic manipulations in mice ii. For dna: restriction enzyme digestion, ligation and sequencing (cloning, pcr (polymerization chain reaction, southern blot analysis. To confirm if you have transcription or absence of transgene. For rna: rt- pcr, northern blot analysis. For protein: western blot analysis. Neuronal structure: cell body, dendrites and axon: protein synthesis, dna replication, etc, axons sends out information. Synapse components: presynaptic elements (synaptic vesicles), synaptic cleft and postsynaptic site (postsynaptic density- psd, spine) Spine/synapse and learning and memory. Spine/synapse and synaptic plasticity (e. g ltp) formation of new dendritic spine are important for learning and memory important for ltp. Spine/synaptic deficits and mental and neurological disorders (autism, schizophrenia, mental retardation, drug addiction etc. ) Dendritic spine density and morphology is different from normal. Spine and synaptic changes in health and disease. Glutamate binds to postsynaptic glut receptors influx of sodium depolarization of neuron signal transduction to cell body.
