BIOC 3100 Lecture 3: Citric Acid Cycle

• School: University of Windsor
• Department: Chemistry and Biochemistry
• Course: BIOC 3100
• Professor: siyarampandey

Document Summary

Pyruvate dehydrogenase complex and cac reactions take place in the mitrochondria where oxygen is used to generate atp by oxidative phosphorylation: the consumption of oxygen (respiration) depends on the rate of pdc and cac reactions. History: 1930: study of oxidation of glucose in muscle, addition of malonate inhibited respiration (oxygen uptake) Malonate is inhibitor of succinate oxidation to fumerate: 1935: catalytic amounts of succinate, fumerate, malate or oxaloacetate accelerated rate of respiration. Sequence of interconversion: succinate fumerate malate oxaloacetate: 1936: sequence of reaction: citrate cis-asconitase isocitrate keotgluterate succinate, 1937: enzymatic conversion of pyruvate and oxaloacetate to citrate and co 2. Reactions of the citric acid cycle: citrate synthase formation of citroyl coa intermediate. Isocitrate is consumed rapidly by the next step, driving the isomerization reaction in the forward direction: isocitrate dehydrogenase has two isoforms, one that uses nad+ and another that uses. Nadp+ as an electron acceptor: -ketoglutarate dehydrogenase complex with different enzymatic activities (similar to.