Anatomy and Cell Biology 3309 Lecture Notes - Eosin, Acid Dye, Glutaraldehyde
24 Oct 2013
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Histology chapter one gloss over it; don"t memorize. Glutaraldehyde: better fixative, cross links proteins and keeps them in place but have two reactive species. Formaldehyde: single reactive species, an aldehyde and precipitates proteins. Sections of an object and they have all different cross sections in order to put it together. Blood vessels are circular in their roots through tissue. Haemotoxylin basophilic tissue, but the base dye: it is used in the lab, this binds to nucleic acids, rna in general, both nucleic acids bind to this dye. Eosin acidophilic tissue, but the acid dye: this is also used in the lab. H and e: there are nuclei, when you see spaces in a tissue you see artificial spaces because there are no spaces refer to as artifacts. Nucleic acid overall charge is negative with phosphate: the basic dye will be positive so these bind to nucleic acids.
