Anatomy and Cell Biology 4429A Lecture Notes - Lecture 3: Metaphase, Proteasome, Mdm2
12 Jan 2018
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Dogma: folin stated that proteins are very stable and there is no need for them to turnover. So people never felt the need to study their degradation mechanisms. It"s easier to look at o(cid:374)e path(cid:449)a(cid:455), (cid:373)ore tha(cid:374) a(cid:374)other. For e(cid:454)a(cid:373)ple, phosphor(cid:455)latio(cid:374) is eas(cid:455) to observe compared to de-phosphorylation (does not occur easily) Discovered the method of tracking a protein, by using isotopically labelled n. he found that rbcs last 120 days. This meant that proteins must be made and turned over continuously. Inhibitors of lysosomes (chloroquine; anti-malarial) did not have to inhibit all proteolysis. Found covalently bound on proteins, particularly proteins that are being turned over. Gly is bound to an amino group of lys side chain of the target protein. A protein about to be degraded can have up to 20 ubiquitin molecules along its side chains. Ring (really interesting new gene) domain directly passes the ubi from e2 to the target protein.
