Biochemistry 2288A Lecture Notes - Lecture 2: Genetic Code, Consensus Sequence, Sense Strand

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29 Dec 2017

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Bacterial operon structure: one promoter (which drive the expression of all genes, one terminator. Termination signals are the certain sequences on the dna which ends or terminate the process of transcription (the synthesis of mrna which is transcribed). Stop codon is the triplet code of three nucleotides sequences in messenger rna or mrna which do not specify any amino acid and marks the termination of translation i. e. synthesis of polypeptide chain. There are two types of strands in dna: sense strand (the strand that codes for proteins, antisense strand (the template strand that is used to synthesis the rna) The antisense strand of dna is read by rna polymerase from the 3" end to the 5" end during transcription (cid:523)(cid:885)" (cid:887)"(cid:524). The complementary rna is created in the opposite direction, in the (cid:887)" (cid:885)" direction, matching the sequence of the sense strand with the.

Related Questions

Match the following:

1	Region which lies upstream of thetranslation start site contains the Shine Dalgarno sequence
2	Sequence added to the 3' end of thepre-mRNA which affects mRNA stability
3	Establishes the reading frame andspecifies the first amino acid of the protein chain
4	DNA sequence that the transcriptionapparatus recognizes and binds to initiate transcription
5	Expressed sequences of DNA that arenot removed during alternative splicing
6	Signals the end of translation andthe protein molecule
7	Group of three nucleotides that codefor an amino acid
8	Site begins the coding region of thegene and is located 25-30 nucleotides downstream of the TATAbox
9	Non-coding sequences of DNA thatintervene within coding regions of a gene
	10 Ribosome binding site ineukaryotes; functions in the initiation of translation and mRNAstability
11	A sequence which lies downstream ofthe coding region of the gene; determines the location of the 3'cleavage site in the pre-mRNA molecule
12	Sequence of nucleotides at the 3' endof the mRNA that is not translated into protein; affects thetranslation and stability of the mRNA molecule

A.	5' untranslated region
B.	AAUAAA consensus sequence
C.	Promoter
D.	Transcription start site
E.	3' untranslated region
F.	Introns
G.	Initiation codon (AUG)
H.	Poly(A) tail
I.	5' cap
J.	Termination codon (UAA, UGA, UAG)
K.	Exons
L.	Sense codon

ivorymacaw91

1)Which of the following is NOT correct when considering the initiation of transcription in E. coli?

A) The Ï subunit of RNA polymerase makes strong contacts with the -10 region of promoters.

B) Only the -10 region of the promoter is required.

C) DNA melting near the transcription start site is essential for promoter recognition.

D) The phosphodiester bonds that are formed are not stable until about 10 nucleotides have been incorporated.

E) The RNA polymerase contacts about 50 bp of DNA.

2)In bacteria, mRNA, tRNA and rRNA are all transcribed by the same RNA polymerase.(TRUE/FALSE)

Which of the following is the best characterization of the reading frame in gene expression?

Question 5 options:

The reading frame of a gene is set by the first three nucleotides in a gene's promoter.

Every gene has three possible reading frames, but all three specify the same protein.

Every gene has three possible reading frames, and thus encodes three different proteins.

The reading frame of a gene is set by the first three nucleotides at the 5' end of the mRNA.

The reading frame of a gene is set by the first AUG codon near the 5' end of the mRNA.

Which of the following is/are NOT involved in initiating transcription in eukaryotes?

Question 9 options:

transcription factors

start codon

promoter

template strand of DNA

RNA polymerase

The chemical piece of DNA monomers that makes one DNA monomer different from another is:

Question 10 options:

	the sugar
	the position of the hydroxyl group on deoxyribose
	the nitrogenous base
	the position of the bond between phosphate and sugar
	the phosphate group

Biochemistry 2288A Lecture Notes - Lecture 2: Genetic Code, Consensus Sequence, Sense Strand

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