Biochemistry 2280A Lecture Notes - P53, Start Codon, Dna Microarray

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Purine = 2 ring structure, a and g, attach to c1 via n9 position. Pyrimidine = 1 ring structure, c and t, attach via the n1 position. Cg has 3 hydrogen bonds, at has only 2. The anomeric carbon of ribose is in the b-configuration when attached to the nitrogenous base, and are joined in an n-glycosidic bond. Phosphates attach to the c5 by a phosphoester linkage, make the dna negative. The double helix is stabilized by base stacking and base pairing. Dna is 2nm wide and can be very long. Chromatin is a complex of dna and its organizing proteins. Histones have fewer than 200 amino acids, and have many lysine and arginine (which are positively charged), and so interacts well with the negative dna. Dna wraps around a cluster of 8 histones to make the nucleosome core particle.

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