Biochemistry 2280A Lecture 23: Biochem 2280 Topic 23
28 Feb 2017
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Estimated to be a billion business. See genomes 3 material in the course pack. Aligning the independent sequences into a continuous sequence. Genomic dna library: a collection of cloned dna fragments that represent all of the dna in an organism"s genome. Assume we have the whole human genome, we cleave that with re (this is the case where we have partial digest) to get millions of genomic fragments. Then, we insert all that fragments into plasmid vectors using dna ligase, so we get representations of each of those fragments in the population. Then that population of clones is transformed into e. coli, which then makes up our genomic library: influenza was the first genome sequences. Clicker q: the h flu genome is 2 mill bp. E coli clones and isolate the plasmid and have primers at the end of the plasmid. They get sequenced and gets run on 500 bases in length. Resulting in 12 mill bp of sequences.
