Biochemistry 2280A Lecture Notes - Lecture 23: Craig Venter, Genomic Library, Francis Collins
12 Dec 2017
School
Department
Course
Professor
Document Summary
Craig venter and francis collins genomics a 500 billion business. Classical genome sequencing involves: creating a genomic dna library, many independent sequencing reactions, aligning the independent sequences into a continuous sequence. A collectio(cid:374) of clo(cid:374)ed dna frag(cid:373)e(cid:374)ts that represe(cid:374)t all of the dna i(cid:374) a(cid:374) orga(cid:374)is(cid:373)"s ge(cid:374)o(cid:373)e. Millions of clones: influenza was the first genome sequenced. Purify the dna fragments of dna base pairs. 20,000 clones each representing fragments of the genome. One clone isolate plasmid dna, anneal primer dideoxy sequence ~500 bases. Putting all 25000 sequences together in the correct order. A stretch of dna sequence aligned form independent sequence data that represents a portion of the genome. A computer searches for overlaps between the 25000 independent sequence runs aligned into contigs. You"(cid:448)e ide(cid:374)tified (cid:1005)4(cid:1004) co(cid:374)tigs a(cid:374)d the orga(cid:374)is(cid:373)s ge(cid:374)o(cid:373)e is circular ho(cid:449) (cid:373)a(cid:374)y gaps do you ha(cid:448)e to fill. With 140 contigs you will have 140 gaps in a circular genome.
