Biochemistry 2280A Lecture Notes - Lecture 4: Coiled Coil, Sh3 Domain, Sh2 Domain
14 Dec 2017
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Document Summary
Concerns the 3-d arrangement of a single polypeptide chain: gives overall shape of polypeptide. 3" structure: domains: a critical concept for larger proteins, definition: distinct region of a protein. Domains can often fold independently (folding of domain in yellow doesn"t depend on blue or any other part of protein) therefor domain. Domains provide structure and/or function on their own: many proteins made up of connected domains, linked together by linker regions, related domains often found in different proteins. Example of domains vs. 3" structure: p60src, kinase domain (kinase adds a phosphate onto a substrate, sh3 domain, sh2 domain. Concerns the 3-d arrangement of multiple polypeptide chains within a single protein. Stabilized by same bonds as for tertiary structure: noncovalent, h-bonds. Key ptm: phosphorylation: three amino acids can be phosphorylated all contain a hydroxyl group, the hydroxyl group is where the phosphate is added, now has not one but two negative charges polar uncharged.
