Biochemistry 2280A Lecture Notes - Lecture 15: Non-Homologous End Joining, Reverse Transcriptase, Chromosome

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2280A
• Professor: Derek Mc Lachlin

Document Summary

Initiation: priming, synthesis, proofreading, ligation, replication fork and replication bubble, telomeres, replication inhibitors. Overview of dna replication: replication is semi-conservative. In the new double helix, half of the. Dna has been conserved from the original strand. The other half is new: semi-conservative nature of dna replication was discovered by meselson and stahl in 1958. Initiation: repli(cid:272)atio(cid:374) does(cid:374)"t sta(cid:396)t (cid:396)a(cid:374)do(cid:373)l(cid:455) o(cid:374) dna. Pe(cid:272)ifi(cid:272) lo(cid:272)atio(cid:374)s (cid:272)alled replication origins is where replication will start. I(cid:374) (cid:271)a(cid:272)te(cid:396)ia, it"s eas(cid:455) to see (cid:449)he(cid:396)e the o(cid:396)igi(cid:374) is (cid:271)ased o(cid:374) the k(cid:374)o(cid:449)(cid:374) replication origins. In bacteria, replication starts in one place only. Whole genome gets replicated in that one place only; goes around the circle. In humans, we have a much more complicated genome much bigger and divided into multiple linear chromosomes: we need at least o(cid:374)e (cid:396)epli(cid:272)atio(cid:374) o(cid:396)igi(cid:374) o(cid:374) ea(cid:272)h (cid:272)h(cid:396)o(cid:373)oso(cid:373)e, (cid:271)ut that"s (cid:374)ot even enough. In fact, eukaryotes have many thousands of replication origins (30-