Biochemistry 2280A Lecture Notes - Lecture 4: C-Terminus, N-Terminus, Protein Folding
Document Summary
Driving force behind protein folding into tertiary structure: entropy, the driving force is hydrophobic interactions, it hates interacting with water. They all gather together and minimize their extent to associate with water: this happens when protein folds, the side chains are all distributed in the unfolded polypeptide, but nonpolar side chains are hydrophobic. Irreversible denaturation: the unfolding is irreversible, add urea to the protein, gradually unfold the protein, it will refold, folding is driven by the sequence of protein. It is a spontaneous process: cooking an egg, the protein comes out of the solution. They precipitate: the major of protein of egg white is called albumen. It has lots of cysteine residue in it: they are not present as disulfide bond, when you heat up the egg, the protein unfolds. Disulfide bonds can occur because cysteine is not at where they normally supposed to be: crosslinking occurs.