Biochemistry 2280A Lecture Notes - Lecture 12: Alternative Splicing, Exonuclease, Polyadenylation

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In bacteria, transcription and translation happen simultaneously; once the rna starts being synthesized, ribosomes bind and begin translation. In eukaryotes, transcription must go to completion before the mrna can leave the nucleus and go to the ribosomes in the cytoplasm to being translation. Importance of the 5" cap and 3" polya tail: mark the 5" and 3" ends of the mrna as being in tact, which is required for, mrna export from the nucleus, translation, protect the mrna from degradation, splicing: In eukaryotes, protein coding sequences called exons can be interrupted by non- coding sequences called introns: exons are expressed, introns are interruptors. Introns provide the opportunity for differential splicing; the mrna can be put together in different ways by leaving out certain exons to create multiple functionally related but distinct proteins. Introns are removed in 2 consecutive transesterification reactions.

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